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Antibody(anti-tag antibodies)|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody(anti-tag antibodies)

【B】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
beta-gal 2011-11-26 40-1AWBIF IHCIP
Developmental study hybridoma bank Sx100
4%PFA +0.1%Triton
mouse IgG1
IgG1

Drosophila embryo, discs, nervous system
be-ta Gal2011-11-26 WBIF IHCIP
cappel
55976
4%PFA +0.1%Triton
rabbit

Drosophila embryo, discs, nervous system

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
CAT2011-08-14 WBIF IHCIP
Boulder
5307-310127
rabbit
p

【D】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
DDDDK (epitope of flag tag)2011-09-27 WBIF IHCIP
MBL200-500
PM020
rabbit
p
1%PFA, 4%PFA, MeOH, TCA
DDDDK2011-10-28 WBIF IHCIP
MBL1/1000
PM020
rabbit
p

【F】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
FLAG2011-08-29 WBIF IHCIP
Chemicon
mouse
Mono, IgG1

Hela細胞
DYKDDDDK (Flag)2012-08-23
About Product
1E6WBIF IHCIP
和光純薬x2000x2000
018-22381
3%PFA (in PBS)
mouse
0.05%saponin
IgG2b

Hela細胞
FITC2011-08-10 WBIF IHCIP
Zymed
711900
rabbit
p

FLAG2011-08-29
About Product
PolyclonalWBIF IHCIP
Abcam
ab1257
goat
IgG
ダマができる
Hela細胞
FLAG2011-08-29 M2WBIF IHCIP
SIGMAx5000-10000
F1804
mouse
Mono, IgG1

Hela細胞

【f】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Anti-Flag2015-01-13 WBIF IHCIP
Sigmax1000x500
F1804
3%PFA (in PBS)
Mouse
0.05%saponin
IgG

MEF

【F】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Flag M22011-10-03 WBIF IHCIP
Sigmax1000?3000x1000
F3165
mouse
Mono,IgG1

HEK293T細胞,COS7細胞,rat hippocampal neurons
FLAG® M22011-10-17 M2WBIF IHCIP
SigmaX1000X1000
F3165
mouse
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Mono
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞
FLAG2011-10-28 M2WBIF IHCIP
sigma
F3165
mouse
m
IPは非常によい
Very good for IP
FLAG2011-08-29 WBIF IHCIP
SIGMAx200-500
F7425
rabbit
Poly
核小体が染まる、二次抗体 anti-mouse Alexa555及びanti-mouse Cy3がanti-FLAG (poly)を認識する可能性あり。要注意
Stains nucleoles.
Hela細胞
FLAG® 2011-10-17 WBIF IHCIP
SigmaX1000X1000
F7425
rabbit
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞
FLAG-biotin2011-08-29 M2WBIF IHCIP
SIGMAx1000
F9192
mouse
Mono, IgG1

Hela細胞
fluoroscein2011-08-10 HRP labeledWBIF IHCIP
Chemicon
MAB045P
mouse
m

【G】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
E.coli b-galactosidase2011-08-10 40-1aWBIF IHCIP
DSHBx100
3%PFA (in PBS)
mouse
cappelのものがベスト
Ab from Cappel is the best for beta-galactosidase antibody
lacZ発現させたPC12細胞
GFP-biotin2011-08-29 600-406-215WBIF IHCIP
ROCKLANDx1000
rabbit
poly

Hela細胞
GFP2011-08-29 WBIF IHCIP
Invitrogen (Molecular Probes)x200x10000
rabbit
Poly

Hela細胞
GFP2011-08-29 JL-8WBIF IHCIP
BD Transductionx1000
mouse
Mono, IgG2a

Hela細胞
GFP2011-10-03 WBIF IHCIP
MBLx1000
rabbit
Poly
メーカー推奨:WB x5000~10000
HEK293T細胞、rat hippocampal neurons
GFP2012-12-11 WBIF IHCIP
Nacalaix500
04404-84
4%PFA (in PBS)
rat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min

mouse brain
GFP2011-10-13 7.1 and 13.1WBIF IHCIP
RocheX2000
11 814 460 001
mouse
Mono, IgG1k

HeLa細胞
GFP2011-10-28 7.1/13.1WBIF IHCIP
roche1/10001/1000
11814460001
1%FA
mouse
m

GFP2011-08-10 GF090RWBIF IHCIP
Nacalai tesquex100
440484
3%PFA (in PBS)
rat
m

GFP transfected cells
b-galactosidase2011-08-10 WBIF IHCIP
MP Biom(cappel)x100
55976
3%PFA (in PBS)
rabbit
cappelのものがベスト
Ab from Cappel is the best for beta-galactosidase antibody
lacZ発現させたPC12細胞
GFP2012-12-11 WBIF IHCIP
MBLx500
598
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG

mouse brain
GFP2013-08-05 WBIF IHCIP
MBLx2,000
598
rabbit
IgG

GFP2013-08-05 JL-8, MonoclonalWBIF IHCIP
TaKaRa (clontech)x2,000
632380
mouse
IgG2a

GFP2011-10-13 JL-8WBIF IHCIP
ClontechX3000
632381
mouse
Mono, IgG2a

HeLa細胞
GFP monoclonal2011-08-10 WBIF IHCIP
CLONTECHx100
8362-1

GFP2012-12-11 WBIF IHCIP
Molecular probesx500
A11120
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min

mouse brain
GFP2011-08-10 WBIF IHCIP
Molecular Probesx100
A6455
3%PFA (in PBS)
rabbit

GFP transfected cells
GFP-biotin2011-08-29
About Product
MonoclonalWBIF IHCIP
Abcam
ab291
mouse
IgG1

Hela細胞
β-Galactosidase2011-08-10 GAL40WBIF IHCIP
Sigmax100
G6282
3%PFA (in PBS)
mouse
m
cappelのものがベスト
Ab from Cappel is the best for beta-galactosidase antibody
lacZ発現させたPC12細胞
GST2011-08-29 WBIF IHCIP
SIGMAx2000
G7781
rabbit
Poly

Hela細胞
GFP2012-12-11 WBIF IHCIP
Avesx1000
GFP-1020
4%PFA (in PBS)
chicken
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgY

mouse brain
GFP2011-08-10 clone: 1E4WBIF IHCIP
MBLx100
M048-3
3%PFA (in PBS)
mouse
m

GFP transfected cells
GST2011-09-29 B-14WBIF IHCIP
Santa Cruzx1,000
sc-138
mouse
mono,

GST2011-08-10 Z-5WBIF IHCIP
Santa Cruz
sc459
rabbit
p

E.coli b-galactosidase2011-08-10 WBIF IHCIP
Promegax100
Z378A
3%PFA (in PBS)
mouse
m
cappelのものがベスト
Ab from Cappel is the best for beta-galactosidase antibody
lacZ発現させたPC12細胞

【H】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
His2011-08-29 H-15WBIF IHCIP
Santa Cruz
rabbit
Poly
Lot. J288
Hela細胞
His2011-08-29 H-15WBIF IHCIP
Santa Cruz
rabbit
Poly
Lot. F1803
Hela細胞
HA2011-08-29 sc-807WBIF IHCIP
Santa Cruz
rabbit
poly

Hela細胞
HA112011-08-29 WBIF IHCIP
BAbCOx1000
rabbit
Poly

Hela細胞
HA112011-08-29 WBIF IHCIP
MBLx2000
rabbit
Poly

Hela細胞
HA2011-08-29 3F10WBIF IHCIP
Rochex1000x200
rat
Mono, IgG1

Hela細胞
HA2011-08-29 12CA5WBIF IHCIP
Rochex1000x200
mouse
Mono, IgG2b

Hela細胞
HA2011-10-13 3F10WBIF IHCIP
RocheX500
rat
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
Mono, IgG

HeLa細胞
HA2011-10-13 12CA5WBIF IHCIP
Roche
11 583 816 001
mouse
Mono, IgG2bK

HeLa細胞
HA high affinity2011-08-10 3F10WBIF IHCIP
Rochex100
1867423
3%PFA (in PBS)
rat
m

HA transfected cells
HA2013-08-05 3F10, monoclonalWBIF IHCIP
Rochex2,000
1867423
rat
IgG1

HA2011-10-28 WBIF IHCIP
roche1/10001/250
1867431
1% FA or 100% MeOH
rat

His2011-08-10 WBIF IHCIP
Amersham B
27471001
mouse
m

HA2011-08-29
About Product
MonoclonalWBIF IHCIP
Abcam
ab18181
mouse
IgG3
Whole HA抗体
Antibody for whole HA molecule
Hela細胞
HA2011-10-13 16B12WBIF IHCIP
covanceX200X500
MMS-101P
mouse
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
Mono, IgG1

HeLa細胞
HA2013-08-05 16B12, monoclonalWBIF IHCIP
Covancex2,000
MMS-101R
mouse
IgG1

【M】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Myc2011-08-29 WBIF IHCIP
MBLx100x100
rabbit
Poly

Hela細胞
c-Myc2011-08-29 9-E10WBIF IHCIP
Santa Cruz (BabCO)x1000x1000x500
mouse
Mono, IgG1
Lot. G050
Hela細胞
c-Myc2011-08-29 9-E10WBIF IHCIP
Santa Cruz (BabCO)x1000x1000x500
mouse
Mono, IgG1
Lot. G2803
Hela細胞
myc2011-10-03 PL14WBIF IHCIP
MBLx1000
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○
Mono,IgG1

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
COS細胞,rat hippocampal neurons
Myc-Tag2011-08-14 9B11WBIF IHCIP
Cell Signalingx1000x100
2276
mouse
m

【m】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Myc-Tag2015-01-13 WBIF IHCIP
Cell Signaling Technologyx2000
2276
3%PFA (in PBS)
Mouse
0.05%saponin
IgG2a

MEF

【M】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
myc2011-10-03 WBIF IHCIP
MBLx1000x1000
562-5
rabbit
Poly

HEK293T細胞,COS細胞,rat hippocampal neurons
c-Myc(9E10)2011-10-17 WBIF IHCIP
Santa CruzX500X500
sc-40
mouse
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Mono
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞

【N】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
neomycin phosphotransferase II2011-08-10 WBIF IHCIP
Upstatex1000
06747MN
rabbit
p

マウス脳

【R】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
RFP2011-08-29 8D6WBIF IHCIP
MBL
mouse
Mono, IgG1
良くない、WBにはPolyclonalを使うこと
Not good. Use polyclonal Ab for WB
Hela細胞
RFP2011-08-29 3G5WBIF IHCIP
MBL
mouse
Mono, IgG1
IP用
For IP only.
Hela細胞
RFP2011-08-29 WBIF IHCIP
MBLx1000x1000
rabbit
Poly
mCherryに使える
Also applicable for staining mCherry.
Hela細胞

【T】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
T72011-08-29 69522-3WBIF IHCIP
novagenx10000
mouse
Mono, IgG2b

Hela細胞

【V】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
VSVG2011-08-29 P5D4WBIF IHCIP
SIGMA
V5507
mouse
Mono, IgG1

Hela細胞

【X】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Xpress2011-08-29 WBIF IHCIP
Invitrogenx5000x1000x800
mouse
Mono, IgG1

Hela細胞